2005). Thus, many studies have evaluated the effects of
anti-inflammatory agents on the direct inhibition of NO production
(Chan, Ho, & Huang, 1995). The anti-inflammatory effects
of MJ treated and untreated strawberry crude extracts on
the NO production on cells exposed to LPS in a concentration
of 25 and 50 lg/mL were evaluated (Fig. 1). First, the effect of
the extracts on murine macrophages viability was
examined. The treatment of the extracts did not show any
significant cytotoxic effect in the present experiments (data
not shown). When control cells were exposed to LPS the
amount of NO increased by four times. A dose of 25 and
50 lg/mL of the MJ untreated strawberry crude extract did
not decrease the LPS-induced NO production significantly.
MJ treated strawberry crude extract was able to
significantly (P > 0.05) attenuated LPS-induced NO production
at 50 lg/mL.
The four fractions obtained fromthe MJ treated strawberry
crude extract were also evaluated in this assay at 25 and
50 lg/mL (Fig. 2). The effect of the fractions on cell viability
of murine macrophages was tested. The treatment of strawberry
fractions did not show any significant cytotoxic effect
in the present experiments (data not shown)