2.2. Preparation of extracts
The endophytes were grown in potato dextrose broth
media for 10 d at 27 °C on a shaker at 160 r/min. The
mycelium were filtered and dried. The dried powdered
materials were extracted with organic solvent ethyl acetate
(1:10) by using cold percolation for 48-72 h. The obtained
extracts were filtered by using Whatman No. 1 filter paper
and then concentrated under vacuum at 40 °C by using a
lyophilizer.