One of the most used immobilisation methods is the entrapment of cells in gel matrices. Due to the mass transfer limitation in this matrix the lipase productivityis often limited. To prevent this problem a natural immobilisation techniqe was employed in this study.The cells were immobilised by physical entrapment in the open pore network of reticulated polyurethanefoam. This natural immobilisation technique has several advantages over the other methods. Having giventhe important advantages that any immobilised biocatalyst may offer a priori, the present work has focused its interest in fermentation conditions for lipaseproduction