Egg albumen was separated from the yolks and stirred for 3 minutes without forming foam, ultraviolet sterilized by placing it on a portable clean room (PCR) hood (UV Sterilisation Cabinet) (GLE-UVSC-in version 09-05,22 Cambridge Science Park, Milton Road, Cambridge, UK) for 15 minutes. The working culture was added and homogenized with a tube shaker, then fermented at 37°C incubator (Ecocell LSIS-B2V/EC III SERIAL D 112456, MMM Medcenter Einrichtungen GmBH Semmelweisstrasse 6, Planegg, Germany) according to the duration of fermentation in the treatment.