To study the effect of formation of TFAs in heated/reheated fats/
oils used for frying, a similar process as described above was carried
out, wherein, a karahi containing 500 mL of the fat/oil sample
was taken, and, after the initial heating-up process and maintaining
the fat/oil temperature at 180 C for 30 min, a first frying cycle
was started. During each frying cycle, 50 g PFFF were fried. The
subsequent frying cycle was performed after continuing heating
and maintaining the same oil sample for half an hour at 220 C,
and then sample was allowed to cool for a period of 60 min.