The next part of the examined
samples were carp and koi carp for export from professional breeders, imported koi carp and fish from breeds
with increased mortality (45 and 21 fish in 2005 and 2006, respectively) The only virological testing was done in
this case. The culture and PCR method, according to Gilad et al. (2002) showed negative results for virus KHV
in all years. Retrospective investigation by PCR method according to Bercovier et al. (2005) showed positivity
in five locations in 2005. In 2006, KHV virus was not detected in any of the selected locations. The aim of our
study was to find out the presence/absence of KHV in selected locations and potential correlation of virological,
bacteriological and haematological findings. The results of first testing for presence of koi herpesvirus indicates
the necessity of regular KHV monitoring in the Czech Republic in the next period.