The qRT-PCR was conducted using Realplex Mastercycler
(Eppendorf, Hamburg, Germany) with a reaction volume of 25 μl,
containing 1.0 μl of the first-strand cDNA, 0.5 μM each of the
primers, 12.5 μl of GoTaq qPCR Master Mix (Promega Corp.),
and 10.5 μl of nuclease-free water.