HPLC
analysis of desulphoglucosinolates was carried out using a Waters
HPLC instrument equipped with aModel 2996 PDA absorbance detector
(Waters, USA). Samples (20 μL) were separated at 30 °C on a Waters
Spherisorb C18 column (250×4.6 mm i.d.; 5 μm particle size) using
acetonitrile and water at a flow rate of 1.0 mLmin−1