2.3.1. Proximate composition
Total nitrogen was determined using the Kjeldahl method,
method no. 981.10 (AOAC, 2005) and calculated into protein content
(N 6.25). Moisture content was determined by drying the
sample in a hot air oven at 100 C until a constant weight was obtained,
method no. 952.45 (AOAC, 2005). Crude fat content was
determined by acid digestion prior to continuous extraction using
petroleum ether in Soxtec system, method no. 945.16 (AOAC,
2005). Ash content was analysed by incinerating all organic matter
at 550 ± 5 C, method no. 945.46 (AOAC, 2005). Carbohydrate per
100 g was calculated using the following formula: 100-moistureprotein-fat-ash;
energy was calculated by Atwater factor (4 for
protein and carbohydrate and 9 for total fat). Total dietary fibre
was analysed using the enzymatic gravimetric method, method
no. 991.43
2.3.1. Proximate compositionTotal nitrogen was determined using the Kjeldahl method,method no. 981.10 (AOAC, 2005) and calculated into protein content(N 6.25). Moisture content was determined by drying thesample in a hot air oven at 100 C until a constant weight was obtained,method no. 952.45 (AOAC, 2005). Crude fat content wasdetermined by acid digestion prior to continuous extraction usingpetroleum ether in Soxtec system, method no. 945.16 (AOAC,2005). Ash content was analysed by incinerating all organic matterat 550 ± 5 C, method no. 945.46 (AOAC, 2005). Carbohydrate per100 g was calculated using the following formula: 100-moistureprotein-fat-ash;energy was calculated by Atwater factor (4 forprotein and carbohydrate and 9 for total fat). Total dietary fibrewas analysed using the enzymatic gravimetric method, methodno. 991.43
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