For detection of avian leukosis
viruses, six pairs of primers were synthesized at
BioBasic Inc., Torbay Road, Markham Ontario, Canada.
The primers were chosen according to published
sequences of the endogenous and exogenous gp85env
gene (Pham et al., 1999 and Smith et al., 1998) (Table
1). Detection of ALV in vaccines by using reverse
transcriptase-polymerase chain reaction (RT-PCR).