This study directly compared the dynamics of change in plasma corticosterone concentration
with the dynamics of change in tissue corticosterone concentration in laying hens. In
concert, we measured the rate of gluconeogenesis, glycogenesis, and glycolysis in the liver,
kidney, skeletal muscle, and heart.We evaluated these changes acutely, over 3 h in response
to an adrenocorticotropic hormone (ACTH) injection, and chronically, over 24 h in response
to food and water deprivation. In response to ACTH injection, there was a significant (P <
0.05) increase in plasma corticosterone concentration and a parallel significant increase in
corticosterone concentration in the skeletal muscle, kidney, and heart. However, the change
in corticosterone concentration in the liver did not parallel the plasma, at times it was
greater than the plasma, and there was a second significant increase (P < 0.05) in corticosterone
concentration in the liver after 180 min. Under these conditions, the rate of gluconeogenesis
in the liver decreased and the rate of glycogenesis increased. In contrast, after
12 h and 24 h of food and water deprivation plasma corticosterone concentration was
increased, and this was paralleled by increased corticosterone concentration in the liver, an
increase in the rate of gluconeogenesis and a decrease in the rate of glycogenesis. After ACTH
injection, glucose concentration in the liver was not significantly depleted but after 12 h or
24 h of food and water deprivation it was significantly depleted (P < 0.05)
This study directly compared the dynamics of change in plasma corticosterone concentrationwith the dynamics of change in tissue corticosterone concentration in laying hens. Inconcert, we measured the rate of gluconeogenesis, glycogenesis, and glycolysis in the liver,kidney, skeletal muscle, and heart.We evaluated these changes acutely, over 3 h in responseto an adrenocorticotropic hormone (ACTH) injection, and chronically, over 24 h in responseto food and water deprivation. In response to ACTH injection, there was a significant (P <0.05) increase in plasma corticosterone concentration and a parallel significant increase incorticosterone concentration in the skeletal muscle, kidney, and heart. However, the changein corticosterone concentration in the liver did not parallel the plasma, at times it wasgreater than the plasma, and there was a second significant increase (P < 0.05) in corticosteroneconcentration in the liver after 180 min. Under these conditions, the rate of gluconeogenesisin the liver decreased and the rate of glycogenesis increased. In contrast, after12 h and 24 h of food and water deprivation plasma corticosterone concentration wasincreased, and this was paralleled by increased corticosterone concentration in the liver, anincrease in the rate of gluconeogenesis and a decrease in the rate of glycogenesis. After ACTHinjection, glucose concentration in the liver was not significantly depleted but after 12 h or24 h of food and water deprivation it was significantly depleted (P < 0.05)
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