shoot tips can result in virus-free plants at a high frequency.
Thermotherapy had other effects which together
with subsequent cryotherapy resulted in virus
elimination. When used for cryotherapy, conditions
are selected to allow survival of only a limited number
of the least differentiated cells and to eliminate a large
proportion of virus-infected tissues. Therefore, cryotherapy
can result in virus-free regenerants with a much
greater frequency than what is typically obtained with
a conventional meristem culture (Wang and Valkonen,
2008). Cryotherapy has successfully eliminated pathogens
(viruses and bacteria) from numerous plant species:
Solanum tuberosum, Ipomea batatas, Vitis vinifera,
Citrus spp.,
shoot tips can result in virus-free plants at a high frequency.Thermotherapy had other effects which togetherwith subsequent cryotherapy resulted in viruselimination. When used for cryotherapy, conditionsare selected to allow survival of only a limited numberof the least differentiated cells and to eliminate a largeproportion of virus-infected tissues. Therefore, cryotherapycan result in virus-free regenerants with a muchgreater frequency than what is typically obtained witha conventional meristem culture (Wang and Valkonen,2008). Cryotherapy has successfully eliminated pathogens(viruses and bacteria) from numerous plant species:Solanum tuberosum, Ipomea batatas, Vitis vinifera,Citrus spp.,
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