Sequence Alignment
Nucleotide sequences of all candidate alleles between HLO32 and HLO30 with National Center for Biotechnology Information GenBank accession number are shown in Figure 2. Sequences of DRB*51, *15, and *22 (resistant alleles) were compared with DRB*1 and *52 (susceptible alleles). Five mutation points were found that could be used to distinguish between resistant and susceptible alleles. Specifically, mutation points were observed at positions 67 (A → C), 200 (G → T), 208 (C → G), 212 (C → G), and 236 (G → T).