The aim of this study was to compare the biogenic amine production of two starter strains of Lactococcus
lactis subsp. cremoris (strains from the Culture Collection of Dairy Microorganisms – CCDM 824 and
CCDM 946) with decarboxylase positive activity in a model system of Dutch-type cheese during a
90-day ripening period at 10 C. During ripening, biogenic amine and free amino acid content, microbiological
characteristics and proximate chemical properties were observed. By the end of the ripening period,
the putrescine content in both samples with the addition of the biogenic amine producing strain
almost evened out and the concentration of putrescine was >800 mg/kg. The amount of tyramine in
the cheeses with the addition of the strain of CCDM 824 approached the limit of 400 mg/kg by the end
of ripening. In the cheeses with the addition of the strain of CCDM 946 it even exceeded 500 mg/kg. In
the control samples, the amount of biogenic amines was insignificant.