Two dominant AM fungi i.e. Glomus mosseae and Acaulospora laevis were isolated from the rhizosphere soil of cluster bean plants by ‘Wet sieving and decanting technique’ of Gerdemann and Nicolson (1963). The starter inoculum of pure culture of each selected dominant AM fungus was raised by “Funnel Technique” of Menge and Timmer (1982) using wheat as host for two months.