Preparative TLC was performed on a 10 × 10 cm silica gel 60 TLC plate with the lipids extracted from a 500 mL culture of strain CD630 spread on a line at the origin. The lipids were chromatographed in Solvent A and all but the furthest left material was scraped in 1 cm bands which were eluted as described above for extraction of cellular lipids. The leftmost materialwas stained for phospholipidswith molybdate reagent and charred.