Examine the DAPI-stained slide in an epifluorescent microscope using UV filter block with 340-380 excitation and 430-450 emission wavelengths. Write down coordinates of the best mitotic spreads and observe the quality of cells in terms of the cytoplasm remaining and the cell flatness. Capture images of well spread metaphase chromosomes at 100x objective lens with Nikon Digital Camera DXM1200F for chromosome analysis