Those concentrations were selected
based on previous studies (Szerman et al., 2008; Vaudagna
et al., 2008). The antifoam was added to the brines at 0.15% (w/v).
The temperature of brines was 1.5±0.5 °C. The brine pH was different
according to its formulation: 6.91±0.06 for MWPC+NaCl,
6.92±0.05 for WPC+NaCl, 8.07±0.14 for NaCl and 8.13±0.07
for STPP+NaCl. Muscles, at a temperature of 3.0±1.0 °C, were
injected to 130% over original weight (measured before the PreIT). In
order to achieve this injection level, muscles were injected once, and
then turned over and re-injected. The injection pressure was between
100 and 200 kPa, depending on the viscosity of the brine, and the
number of strokes was 30 per minute.