The second-stage PCR amplification profilewas as follows: 40 94 C for 40 s, followed by 65 C for 40 s, 72 C for 40 s, and a 5 min final extension at 72 C.
The second-stage PCR amplification profilewas as follows: 40 94 C for 40 s, followed by65 C for 40 s, 72 C for 40 s, and a 5 min final extension at 72 C.