2.2. Isolation and identification of PGPR
Bacterial libraries were prepared from PGPRs collected from the
roots of plants growing in the rhizoplane and rhizosphere of
diverse environments such as coasts, hills, and roadsides as follows.
The roots were washed with sterile water and ground to powder by
using a mortar and pestle. Subsequently, 1 g of the powder was
mixed with 9 mL of sterile saline water and stirred for 30 min at