Most of ethanol was produced at the beginning day as shown
in Fig. 7. The ethanol concentration increased from 45.3 g/L to
222 g/L after one day of cultivation at 33 ◦C, 227 g/L after 4 days and
258.2 g/L after 7 days. The ethanol production increased within 1
day due to a simultaneous saccharification and fermentation process
but beyond that there was a negligible increase or decrease in
ethanol amount. This trend of ethanol production is partially supported
from the CFU data (given in Table 3) that the number of
viable yeast cells becomes much lower or even completely vanishes.