A two-stage process using aqueous ammonia and hot-water has been investigated to
fractionate corn stover. To maximize hemicelluloses recovery and purity in the liquid
hydrolyzate by optimizing the fractionation process, the experiments were carried out
employing response surface methodology (RSM). A central composite design (CCD) was
used to evaluate and confirm the effectiveness and interactions of factors. The optimal
fractionation conditions were determined to be as follow: (1) First-stage reactor operated in
batch mode using a 15% NH4OH solution (wNH3 ¼ 15%) at 1:10 solid:liquid ratio, 60 C, and
24 h; (2) second stage percolation reactor operated using hot-water at 20 cm3 min1, 200 C,
and 10 min.
The model predicted 51.5% xylan recovery yield and 82.4% xylan purity under these
conditions. Experiments confirmed the maximum xylan recovery yield and purity were
54.7% and 83.9% respectively under the optimal reaction conditions.
With the solids resulting from the two-stage treatment, 87%e98% glucan digestibilities
were obtained with 15 FPU of GC 220 per g-glucan and 30 CBU of Novo 188 per g-glucan
enzyme loadings. Xylan digestibility of xylooligomer hydrolysates reached 76% with 8000
GXU per g-xylan of Multifect-Xylanase loading. In the simultaneous saccharification and
fermentation (SSF) test using treated solids and Saccharomyces cerevisiae (D5A), 86 % to 98%
of ethanol yield was obtained on the basis of the glucan content in the treated solids
A two-stage process using aqueous ammonia and hot-water has been investigated tofractionate corn stover. To maximize hemicelluloses recovery and purity in the liquidhydrolyzate by optimizing the fractionation process, the experiments were carried outemploying response surface methodology (RSM). A central composite design (CCD) wasused to evaluate and confirm the effectiveness and interactions of factors. The optimalfractionation conditions were determined to be as follow: (1) First-stage reactor operated inbatch mode using a 15% NH4OH solution (wNH3 ¼ 15%) at 1:10 solid:liquid ratio, 60 C, and24 h; (2) second stage percolation reactor operated using hot-water at 20 cm3 min1, 200 C,and 10 min.The model predicted 51.5% xylan recovery yield and 82.4% xylan purity under theseconditions. Experiments confirmed the maximum xylan recovery yield and purity were54.7% and 83.9% respectively under the optimal reaction conditions.With the solids resulting from the two-stage treatment, 87%e98% glucan digestibilitieswere obtained with 15 FPU of GC 220 per g-glucan and 30 CBU of Novo 188 per g-glucanenzyme loadings. Xylan digestibility of xylooligomer hydrolysates reached 76% with 8000GXU per g-xylan of Multifect-Xylanase loading. In the simultaneous saccharification andfermentation (SSF) test using treated solids and Saccharomyces cerevisiae (D5A), 86 % to 98%of ethanol yield was obtained on the basis of the glucan content in the treated solids
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