In this present study, 307 food handlers working in catering services in Mekelle University students’ cafeteria were undergone screening program for enteric pathogens. Stool cultures obtained from the participants revealed the prevalence of S. typhi (1%) and intestinal parasites (52.4%). So, we concluded as the high preva- lence of intestinal parasites among food handlers in the present study indicates the poor hygiene practice of the food-handlers working in catering establishments at the study site. Such infec- ted food handlers can contaminate food items and drinks that serve in the cafeteria will act as source of infection to consumers via food chain. Based on these findings in this study, the following recom- mendations are prescribed for the control of enteric pathogens: (1) Good personal hygiene and a standard procedure for handling food item should be practiced by the food handlers. (2) Sustainable health education and training programs should be given for food- handling personnel to improve hygienic practices. (3) Regular medical check-up should be an advantage for identifying the health status of the food handlers.