Irritant action on the gastric mucosa according to Komatsu The ulcerogenic effect was determined by the method devised
by Komatsu et al. [11]. The tested compounds were administered to fasted rats having free access to drinking water. Twenty-four hours after administration of the compounds, the rats were sacrificed, the stomach was removed and, after incision along the lesser curvature, rinsed with a tap along with a warm (37 8C) saline soak, then spread on a cork board and pinned down. The mucosa of the glandular part of the stomach was inspected using a binocular microscope (10-fold magnification). The mucosal lesions were evaluated using the five-point scale (0 – no lesions, 1 – erythema, 2 – punctiform ulcers, 3 – small ulcers, 4 – large ulcers, 5 – perforation).