In this study, various pretreatment methods (autoclaving, ultrasound, microwave and steam explosion) have
been compared to determine the most efficient method for the extraction of lipids from three different samples
of microalgae (Nannochloropsis gaditana, Chlorella sorokiniana and Phaeodactylum tricornutum). Among the
studied methods, steam explosion gave the highest lipid extraction yields for the three microalgae species.
Therefore, the method was further studied and the application of acid catalysed steam explosion pretreatment
was investigated for simultaneous lipid extraction and sugar release from microalgae. The effect of different
variables, including temperature and acid concentration, was analysed. The experimental results demonstrate
the efficacy and feasibility of the acid catalysed steamexplosion pretreatment, followed by n-hexane lipid extraction.
Remarkable sugar yields up to 96% were achieved under the pretreatment conditions of 1.7% sulphuric acid
concentration and a temperature of 150 °C during steam explosion. Besides, this study verified high efficiencies
in the extraction of lipid of exploded microalgae using n-hexane against the low efficiencies obtained for the
untreated microalgae.
In this study, various pretreatment methods (autoclaving, ultrasound, microwave and steam explosion) have
been compared to determine the most efficient method for the extraction of lipids from three different samples
of microalgae (Nannochloropsis gaditana, Chlorella sorokiniana and Phaeodactylum tricornutum). Among the
studied methods, steam explosion gave the highest lipid extraction yields for the three microalgae species.
Therefore, the method was further studied and the application of acid catalysed steam explosion pretreatment
was investigated for simultaneous lipid extraction and sugar release from microalgae. The effect of different
variables, including temperature and acid concentration, was analysed. The experimental results demonstrate
the efficacy and feasibility of the acid catalysed steamexplosion pretreatment, followed by n-hexane lipid extraction.
Remarkable sugar yields up to 96% were achieved under the pretreatment conditions of 1.7% sulphuric acid
concentration and a temperature of 150 °C during steam explosion. Besides, this study verified high efficiencies
in the extraction of lipid of exploded microalgae using n-hexane against the low efficiencies obtained for the
untreated microalgae.
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