The egg yolk hydrolysis can be one of indicator to determine the characteristic of X. oryzae species. This test is based on the observation that the enzyme lecithinase can break down the phospholipid emulsion of egg yolk, liberating a turbid zone of free fats around the colonies. The egg emulsion in the agar provides the lecithin to be degraded by the enzyme. A given isolates that contain lecithinase break down the egg yolk and cause clear zones around the colonies [16].