A spectrophotometer is an instrument which is used to measure the intensity of electromagnetic radiation at different wavelengths. Important features of spectrophotometers are spectral band with and the range of absorption or reflectance measurement. Spectrophotometers are generally used for the measurement of transmittance or reflectance of solutions, transparent or opaque solids such as polished gases or glass. They can also be designed to measure the diffusivity on any of the listed light ranges in electromagnetic radiation spectrumthat usually covers around 200 nm-2500 nm using different controls and calibrations.