The enumeration of particular microbial groups was performed by using the following media and
culture conditions: a) Nutrient Agar for total aerobic plate count, pour-plated and incubated at 30 C for 48 h, and also for total aerobic psychrotrophic count, pour-plated and incubated at 10 C for 10 days;
b) MEA for yeasts and moulds, pour-plated and incubated at 25 C for 5 days;
c) VRBD agar for total enterobacteria, pour-plated, overlaid with the same medium and incubated aerobically at 37 C for 48 h;
d) MRS agar for lactic acid bacteria, pourplated and incubated at 25 C for 5 days. The counts were performed
in triplicate.