Simple Summary: In the context of backyard poultry raising, a notable concern arises regarding the susceptibility to parasitic infections. The poultry industry holds a crucial position in ensuring food safety and nutritional requirements, emerging as the most rapidly advancing agricultural sub- sector. The aim of our study was to assess the prevalence of gastrointestinal parasites in chickens raised in the backyard system within the northwestern and central regions of Romania. Fecal samples were collected and tested using flotation, McMaster, and PCR (polymerase chain reaction) methods. The overall prevalence of infection with gastrointestinal parasites was 53.1%. Intestinal parasites demonstrate a pronounced prevalence within the context of backyard poultry flocks, and the substantial burdens imposed by these parasites can deleteriously influence both avian productivity and economic considerations.