Materials and Methods. Cell culture and methylene blue assay. Cancer cell lines were purchased from
American Type Cell Culture (ATCC) and incubated in a humidified atmosphere of 5% CO2 incubator at 37
°C. All culturing work was handled using sterile techniques. Cancer cell cytotoxicity was measured by
methylene blue assay [29] which was carried out 3 days after BJL extract and cisplatin treatment.