Introduction: Research laboratory is the high risk workplace that may harm to health and safety of the operators .The hazard in research laboratory came from chemical, biological substance, electricity and machine &equipment According to the activities on education and research, King Mongkut’s University of Technology Thonburi (KMUTT) uses more than 1,000 types of chemicals in their activities and half of them are hazardous chemical. Chemical in the university has the potential to harm the health and safety risk for all operators in research laboratory. Proper health and safety management will minimize the risk to students, employees and members of the public and reduce the risk of hazardous chemical release to the environment. Health and Safety management has been developed within KMUTT and aim to play an important role to maintain a safe and healthy for all along with environmentally responsible. The purpose of this study is to develop the appropriated Health and Safety management system for research laboratory operator for risk prevention, risk control and safety for all research laboratory operators.