The ion source temperature was kept at 250 C. For quantification of the peak area, the quantitative masses were 247 + 329 + 368 + 458 for cholesterol trimethylsilyl ether (Che-TME), and 215 + 306 + 445 + 460 for cholestanol trimethylsilyl ether (Cha-TME). The data was processed using LECO Corp’s Chromatography TOF software version 4.43.3.0 optimized for Pegasus 4D