Two culture media were employed in the present study: YPS
proliferation medium (yeast extract 10 g/L, peptone 10 g/L and
sucrose 50 g/L) for reactivation and propagation of yeasts; and YPS
fermentation medium (yeast extract 10 g/L, peptone 10 g/L and
sucrose 250 g/L) for fermentation [11]. Additionally, sugarcane
molasses was evaluated after collecting sugarcane molasses from
local sugarcane mills (Tucuman, Argentina) and diluted to achieve
25% of total reducing sugars (TRS). All these media were sterilized
in autoclave at 121 C during 15 min. Agarized YPS proliferation
medium, used to isolate yeasts, was prepared with YPS proliferation
medium and 15 g/L agar.
Twenty-nine strains isolated from sugarcane molasses and
Two culture media were employed in the present study: YPSproliferation medium (yeast extract 10 g/L, peptone 10 g/L andsucrose 50 g/L) for reactivation and propagation of yeasts; and YPSfermentation medium (yeast extract 10 g/L, peptone 10 g/L andsucrose 250 g/L) for fermentation [11]. Additionally, sugarcanemolasses was evaluated after collecting sugarcane molasses fromlocal sugarcane mills (Tucuman, Argentina) and diluted to achieve 25% of total reducing sugars (TRS). All these media were sterilizedin autoclave at 121 C during 15 min. Agarized YPS proliferationmedium, used to isolate yeasts, was prepared with YPS proliferationmedium and 15 g/L agar.Twenty-nine strains isolated from sugarcane molasses and
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