The sugars were determined from 0.02 mL of the extract used
for amino acid analysis. Glucose, fructose and sucrose contents were analysed by ion chromatography (Metrohm 850 system)
using injection valve along with the Pixel Array Detector (PAD) anion
exchange column (1–150 Metrosep-Carb) and isocratic highpressure
pump for the channel PAD 818 IC. Operating conditions
reported by Moraga, Martínez-Navarrete, and Chiralt (2006) for
performance liquid chromatography were used, with minor modifications
(mobile phase NaOH 80 mM, at 0.9 mL/min flow rate).