Hep G2 cells were treated with or without ACE polysaccharides, ACE/CS and ACE/S in the
same concentrations described above at 37°C for 48 h, trypsinized, washed, resuspended at a
density of 1.0 × 106 cells/mL in PBS and incubated with phycoerythin (PE)-conjugated Fas
monoclonal antibody UB2 (1 pg/mL) at 4°C for 1 h.