To confirm that these negative results were not due to lack of drug efficacy or some systematic problem, positive controls
(P2X2 and GCaMP3.0 or EPAC expressed in the same cell type) were performed concurrently with every
experiment (data not shown). We also did a separate set of experiments using dTrpA1 and a temperature
step to activate DPM neurons, but failed to see any MB calcium responses (data not shown).