The column was ZORBAX SB-C18 and the column temperature was set at 60 °C. Mobile phase was made up of 55% buffer solution (in ultra purified water) and 45% acetonitrile, and flow rate0.5 mL/min. the column temperature was of
The column was ZORBAX SB-C18 and the column temperature was set at 60 °C. Mobile phase was made up of 55% buffer solution (in ultra purified water) and 45% acetonitrile, and flow rate0.5 mL/min.the column temperaturewas of