The present study has been focused on the extraction of natural dyes from Curcuma longa, Trigonellafoenum graecum and Nerium oleander and investigation of their phytochemical and pharmacological char-acteristics. Dyes were prepared using aqueous, acidic, alcoholic and alkaline extraction techniques. UVspectral studies of the dyes showed a variation in absorption maxima and their color varied with respect tothe pH and the solvent used during extraction. The dyes prepared from turmeric using aqueous extractiontechnique and from fenugreek using alkaline extraction showed good antibacterial activity. The aque-ous and alcoholic extraction of Nerium oleander was able to inhibit the growth of many fungal strainsincluding Tricoderma spp., Tricophyton rubrum, Candida albicans, Aspergillus niger, Cladosporium spp. etc.The antimicrobial property of the dyes was used in developing antimicrobial fabric.