Histological changes in black scabbardfish liver. All sections stained with H&E unless otherwise stated. (A) Section of normal liver showing indistinct trabecular
pattern of hepatocytes and occasional hypertrophied hepatocyte nuclei (arrow). Bar ¼ 100 mm. (B) Hepatocellular and nuclear pleomorphism showing enlarged hepatocytes with
loose granular cytoplasm (solid arrow) and hypertrophied nuclei with coarse chromatin (arrows). Bar ¼ 50 mm. (C) Granuloma containing at its centre a collapsed Ichthyophonus cell
and surrounded by a thin layer of flattened epitheloid cells. Bar ¼ 100 mm. (D) Focus of cellular alteration (FCA, arrows). Bar ¼ 0.5 mm. (E) Ovotestis showing foci of spermatocytes
(arrow) amongst ovarian tissue (inset showing detail of spermatocytes stained with Feulgen for DNA). Bar ¼ 50 mm. (F) Normal orange roughy liver showing increased hepatocyte
vacuolation due to lipid storage. Blood vessel (BV). Bar ¼ 100 mm.