Sediment samples collected from the Bhitarkanika mangroves were divided in to two parts as wet sample and dry sample. Serial dilution of wet and dry was done the no of isolates from the dry sample was more in number. Marine sediment samples are good for the isolation of actinomycetes; Goodfellow and Hynes (1984) reviewed the literature on isolation of actinomycetes from marine sediments and suggested that the marine sediment may be valuable for the isolation of novel actinomycetes. 15 isolates of actinomycetes was isolated from the samples. The characterization was done by the phenotypic characterization and species affiliation by physiological and biochemical characteristics described by Das et al. (2008).