The MTH3GH
probe sequence was 5′-CCCATCCTTGGCAATT-3′. Primers designed
from insulin-like growth factor-1 (IGF-1), a constitutively expressed
gene, were used as a genetic control to ensure amplifiable template
was available. Primers used as a genetic control during RT PCR were
IGF1-F forward primer (5′-TGCGATGTGCTGTATCTCCTGTA-3′) and
IGF1-R reverse primer (5′-CCTGTTGCCGCCGAAGT-3′).