Assessment on yield and harvesting loss
Pre-harvest loss was evaluated by five spots randomly
selected in each plot. Each spot was covered with a frame
with 2 m long×50 cm wide, all spontaneously falling seeds
in the frame were collected and weighed and the mean
value of five measured frames were recorded. After each
CH approach, shattering seeds and uncut seeds in the
Fig. PhenotypeChlorophyll
content was detected according to Yuan and Kelly
(1982). A standard germination test (Association of Official
Seed Analysts 1988) was conducted on 100-seed
samples of CH and MH with three replicates at 20°C for 7 d
on moistened blotter papers (Elias and Copeland 2001).