Pineapple propagation by tissue culture. With the use of plant growth regulators or hormones are used to increase the number of new shoots well. In plant tissue culture This growth regulators important and commonly used in the experiments. The hormone cytokinin with a substance that stimulates cell division, cell growth, expanding the size of the branches and trunk. Accelerate the eye and help slow the aging of the plant hormone auxin in the group. Stimulate cell division and growth of cells causes the plant to grow more voluminous . In vitro , the Auxin that are involved with cell division and helps stimulate root (root initiation) and , if applicable, auxin in the volume too high . May be to inhibit the development of cell morphology plant