In the present study, the brine shrimp lethality assay of extracts of two species of Biophytum used in traditional medicine was determined using the procedure of Meyer et al. (1982). The results of the BSLA using the crude ethanol extracts from the different parts of B. veldkampii(Bv) and B. reinwardtii(Br) and details about the percentage mortality and Probit percentage are given below(Tables 3, 4). The LCso values obtained for extracts of these two species and that of the positive control, Potassium Permanganate are given in table 5.