2.7. Assay for phenol and induced defense-related proteins
The enzyme extract from crown tissue of banana was prepared as described by Hwang et al. (1997). The samples from the crown portion of banana fruits treated with plant extracts of Zimmu, Z. scabra, Benomyl and challenge inoculated with L. theobromae and C. musae, uninoculated control (sterile water alone) and inoculated control (inoculated with test pathogens alone) were collected at various time intervals (0, 1, 2, 3 and 4 days after pathogen inoculation) and quickly frozen in liquid nitrogen and stored at 70 C.