Synchytrium endobioticum sporangia pathotypes 1, 2, 6, 8 and 18 were produced susceptible potato sprouts (cv. Deodara) inoculating compost according Spieckermann method described EPPO Diagnostic Protocol for S. endobioticum (Baayen and Stachewicz, 2004) . After 10 week incubation under moist dark
conditions at 16–18 C (dark coloured) were freed adhering soil thorough agitation in
tap water