A novel green sourcewas opted to synthesize silver nanoparticles using dried roasted Coffea arabica seed extract.
Bio-reduction of silver was complete when the mixture (AgNO3 + extract) changed its color from light to dark
brown. UV–vis spectroscopy result showedmaximumadsorption at 459 nm, which represents the characteristic
surface plasmon resonance of nanosilver. X-ray crystal analysis showed that the silver nanoparticles are highly
crystalline and exhibit a cubic, face centered latticewith characteristic (111), (200), (220) and (311) orientations.
Particles exhibit spherical and ellipsoidal shaped structures as observed fromTEM. Composition analysis obtained
from SEM–EDXA confirmed the presence of elemental signature of silver. FTIR results recorded a downward
shift of absorption bands between 800–1500 cm−1 indicting the formation of silver nanoparticles. The mean
particle size investigated using DLS was found to be in between 20–30 nm respectively. Anti-bacterial activity
of silver nanoparticles on E. coli and S. aureus demonstrated diminished bacterial growth with the development
of well-defined inhibition zones.