5.7. Effect of chitosan on S. sclerotiorum rot of carrot
Carrots were wounded (5 mm deep and 3 mm wide) with a
sterile nail at the equator. Then 50 μL of 1% chitosan and
sterile distilled water as the control were placed into each
wound. Carrots were air-dried for 2 h, a mycelial disk was
added to each wound. Treated carrots were put in 200 mm×
130 mm×50 mm plastic boxes with sterile water to maintain
a high relative humidity (about 95%) and stored at 25°C.
Disease incidence and lesion diameter of carrot caused by
S. sclerotiorum was determined each day after inoculation.
Each treatment contained three replicates with 10 carrots
per replicate and the experiment was repeated three times.