Fermentation was carried out in 250 mL Erlenmeyer flask at
30 C and 150 rpm for 24 h with 50 mL of enzymatic hydrolysate
adding 6 g/L yeast extract, 10 g/L peptone, 5 g/L (NH4)2SO4, 10 g/L
KH2PO4, 0.5 g/L MgSO47H2O and 0.15 g/L CaCl2H2O. Each flask
was equipped with a needle-pierced silicone stopper to allow
removal of the produced CO2 during fermentation.