attempted by many researchers [3,7–12]. However, the higher vis- cosity and other physicochemical features of the oils have limited its usage in engines for long term. Since transesterification process of the oil for making esters was found more compatible with the properties of fuel, the esterification process of vegetable oils have been tried in the production of biofuels. The nutritional, therapeutic and industrial qualities of coconut oil, the commercial viability of non lipid components of coconut and the perennial nature of yielding of the coconut palms make the crop unique from other tropical oleaginous crops. The main focus of the previous reports on production of biofuel from coconut oil was the transesterification of the oil to ethyl and methyl esters for blending it with diesel in vehicles [12–14]. An independent search on the standardization of the transesterification of coconut oil with respect to yield of CME, physicochemical properties of CME related to fuel quality and its technical feasibility as biofuel in CIE without any alteration has not been attempted to date scien- tifically. Therefore a detailed analysis of the coconut oil and the esters is inevitable for determining the functional efficacy of CME as biofuel. In the present investigation, an effort was taken to stan-dardize coconut methyl ester (CME) and glycerol production from coconut oil and to analyze the physicochemical features of CME for determining its technical feasibility as biofuel in diesel engine.